An evaluation of alternative methods for sanitizing hatching eggs

ABSTRACT Different sanitization methods were evaluated as alternatives to formaldehyde fumigation for the reduction of eggshell and yolk sac microbiological counts, improvement of eggshell quality, incubation parameters, and day-old chick quality. A total of 10,080 hatching eggs were collected from a 70-wk-old commercial broiler breeder flock and distributed in a completely randomized block design with seven treatments: fumigation with paraformaldehyde (5.03 g/m3/30 min), fumigation with ozone (5–15 ppm/30 min), ultraviolet light-C irradiation (8.09 mW/cm2; 120 s; UV-C), hydrogen peroxide spraying (3%; 0.69 mL/egg), peracetic acid spraying (0.3%; 0.69 mL/egg; PAA), water spraying (0.69 mL/egg; water control), and without disinfection (dry control—DC). Spraying eggs with PAA and UV-C significantly reduced aerobic bacteria plate counts compared to the DC group. In addition, eggs disinfected with PAA had lower Enterobacteriaceae counts than the DC and water control groups. Eggshell quality, incubation parameters, and microbiological counts for yolk sac did not differ (P > 0.05) among treatments. This study demonstrated the potential for the application of PAA and UV-C for eggshell disinfection instead of formaldehyde; however, an electronic microscopic evaluation of the eggshell is necessary to determine if these methods cause any damage to the cuticle.