Molecular studies on antibiotic resistant Bacillus cereus isolated from meat products and human in Kaliobia, Egypt
2020
The current study was conducted on 210 random samples of meat products (beef burger, kofta, luncheon, minced meat, sausage) and diarrheic human stool of patients suffering from vomiting and diarrhoea (35 for each). The meat products were collected from different shops and hospitals at Kaliobia Governorate, Egypt, for detection of B. cereus strains, and their phenotypic characterization as well as antibiotic resistant genes. Bacteriological examination of the collected samples indicated the identification of 51 (24.3%) isolates of B. cereus from 210 samples as 11/31.4% from kofta 13/37.1% from minced meat; 9/25.7% from sausage; 7/20.0% from beef burger; 6 /17.1% from luncheon samples; and 5/14.3% from human stool specimens. Most of 51 isolated B. cereus strains had the ability for biofilm production. The antibiotic sensitivity profiles revealed that, the isolated B. cereus were highly resistant for Penicillin-G followed by methicillin; ampicillin; oxytetracycline; sulfa-trimethoprim and cefotaxime. Meanwhile, they were highly sensitive to to gentamycin and norfloxacin followed by ciprofloxacin; meropenem and florphenicol. Further, PCR declared that bla; tetA and erm genes were amplified in 9; 7; 6 out of 10 studied B. cereus isolates giving products of 680 bp.; 502 bp. and 645 bp. respectively. Therefore, one can conclude that; B. cereus especially antibiotic resistances ones are meat-borne pathogens of public health importance and they may be the causative agents in patients suffering from vomiting and diarrhoea.
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