One-step RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for influenza virus

Disclosed is an influenza virus-step RT-PCR detection kit, relates to the field of biotechnology PCR kits. The kit includes a step 5xRT-PCRBuffer, a positive control, a negative control, three pairs of specific primers and EnzymeMix; based on M gene of influenza virus type A design of specific primers, AF: 5'-ATTCTAACCGAGGTCGAAACGT-3 ', AR: 5' -GACAAAGCGTCTACGCTGCAGTC-3 '; the basis of influenza virus type B NS gene specific primers were designed; type a H1N1 influenza virus based on the HA gene specific primers were designed. The present invention is a short measurement time period, a high detection efficiency; detecting high virus-specific, high accuracy; virus can qualitative analysis; detection sensitivity higher than the sensitivity of immunological detection methods; simple, easy to spread; reproducible results.