Antioxidant and cytoprotective effects of enzyme‐extracted constituents of Protaetia brevitarsis seulensis powder

This study was aimed to compare the antioxidant and cytoprotective effects of hot‐water and enzyme‐extracted constituents of Protaetia brevitarsis seulensis powder. The products of enzymatic extraction had 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH)‐radical‐scavenging activity, cytotoxic effects, and provided cytoprotection against H₂O₂ and intracellular reactive oxygen species. Enzymatic extraction improved DPPH‐radical‐scavenging activity relative to hot‐water extraction (P   0.05). Hot‐water extraction achieved greater cytoprotection against H₂O₂ than enzymatic extraction. However, enzymatic extraction achieved greater antioxidant and cytoprotective activity against reactive oxygen species than hot‐water extraction. These results provide evidence of the antioxidant and cytoprotective effects produced by enzymatic extraction of the active ingredients from Protaetia brevitarsis seulensis powder.