Isolation and purity assessment of Lupin albus conglutins.

An increasing demand of Lupinus proteins for feeding as well as the need of isolating large quantities of pure conglutin-γ for nutraceutical purposes prompted us to search for adequate scale preparative and analytical procedures. L albus seeds were dehulled, finely ground and defatted with hexane. Being careful of performing all steps of the procedure at 4°C and centrifuging always at 5000 rpm we performed water extraction of the defatted flour, pooled all water supernatants for vacuum drying and labelled this powder as albumins . Pellets were dissolved in 10% NaCl solution (3X). Their pooled supernatants were mixed with (NH 4)2SO 4 to reach 85% saturation. After