Abstract 2680: Antitumor promoting activity of the phytotherapic Polypodium leucotomos in oral cancer

The oral squamous cell carcinoma (OSCC) is the eighth most prevalent malignant neoplasm and representing an important health problem worldwide. OSCCs have a highly variable clinical course, and due to its late diagnostic in advanced stages, the overall survival rate is less than 50% in 5 years. Despite the advances and last recent discoveries, the prognosis of this disease is still unpredictable. The search for new therapeutic strategies of OSCC is still needed to improve patient survival. From this point of view, oscc treatment and adjuvant methods represent a promising and challenging field for researchers. In the last few years, the use of some types of complementary medicines, such as phytotherapeutic products, has significantly increased among cancer patients. Recently, a phytotherapeutic compound, Polypodium Leucotomos (PL), was particularly introduced on the market for the treatment of inflammatory changes and other skin diseases caused by UV radiation, due to its anti-inflammatory, anti-oxidant and immunomodulatory properties. It is possible that PL also demonstrates antitumor action, and therefore seems a plausible target for further investigation regarding its therapeutic potential on oral cancer. The present study has characterized the biological effects of the extract of PL on proliferation, migration and invasion of oral cancer cell lines; and investigated the antitumor potential of PL in vivo, by treating oral tumors chemically induced on the tongue of Balb/C mice. Our results showed that oral cancer cells proliferations measured by MTT assay has decreased up to 60% and 78% for a period of 48hrs PL treatment for the SSC9 and LN1 cells, respectively. The scratch assay did not demonstrate a significant reduction on the migration potential these cells. As for the invasion assay, we verified that both lineages, but mainly LN1, a more aggressive oscc cell line, diminish its breach capacity, after previous incubation with PL (p Citation Format: Pammela A. Borges, Gabriela S. Teixeira, Nathalia N. Pantarotto, Luan Oenning, Liliane O. Marinho, Lucilene Lopes, Eduardo J. Caldeira, Pablo A. Salvador, Ricardo D. Colleta, Clovis A. Pinto, Gustavo Simoes Augusto, Taize M. Augusto, Carine E. Oliveira, Nilva K. Cervigne. Antitumor promoting activity of the phytotherapic Polypodium leucotomos in oral cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 2680.