Some solubility studies of erythropoiesis regulatory factors (ERF)s

Abstract Erythropoiesis regulatory factors, isolated from the urines of a male patient with paroxysmal nocturnal hemoglobinuria (PNH) and a female patient with an anemia secondary to multiple myeloma (MM), were treated with an ethanol: acetone solution for chemical characterizations. The described treatment appeared to inactivate reversibly an erythropoiesis generating factor (EGF, molecular weight 30,000) and irreversibly an erythropoiesis stimulating factor (ESF 2 mol wt >50,000) from the PNH patient. The inactivated EGF appeared to be reactivated in the presence of several adrenocorticosteroids or batyl alcohol. The treatment with ethanol: acetone did not inactivate EGF or ESF 1 (mol wt 20,000) from the MM patient nor an inhibitor of erythropoiesis (mol wt 10,000).