Isolation and characterization of Bioactive Metabolite from Bacillus safensis VRKK2 for controlling Bacterial blight disease in Cowpea

2020 
AIMS: Isolation and characterization of Bioactive Metabolite from Bacillus safensis VRKK2 for controlling Bacterial blight disease in Cowpea. OBJECTIVES: The primary objective of the study was to describe the biocontrol potential of Bacillus safensis VRKK2 against Bacterial blight disease in Cowpea and improve the yield of the cowpea plant. METHODS: Primarily the rhizobacteria isolated from cowpea Rhizosphere screened for the plant growth promoting traits and further Bioactive Metabolite extracted and purified from PGPR and identified using standard analytical techniques. The purified bioactive metabolite efficacy analyzed in In vitro and In vivo study in host plants. RESULTS: From the initial screening the strain Bacillus safensis VRKK2 found to be potential organism of their IAA concentration found to be maximum of 171.2 microg/ml, phosphate solubilization efficiency found to be maximum of 155 SE also positive for siderophore production, nitrogen fixation assay and ammonia production. Phylogenetic tree results with 99% similarity. Extracted compound showed spots on TLC visualized by UV illuminator, fluorescent band was observed at 0.47 Rf value and tested for their pathogen suppression efficiency against Xanthomonas campestris, purified fractions showed 11mm and 15mm of zone of inhibition against test pathogen and the overall disease severity percentage is 34.75 %. CONCLUSION: This study specifies that the isolated PGPR Bacillus safensis VRKK2 found to be better biocontrol agent against bacterial blight disease in cowpea.
    • Correction
    • Source
    • Cite
    • Save
    • Machine Reading By IdeaReader
    98
    References
    0
    Citations
    NaN
    KQI
    []