Temperature cycle device

Providing a useful temperature cycle apparatus for carrying out the reaction, especially nucleic acid amplification of biological samples to be used in molecular biology research, and the like. The reaction liquid 5 a temperature cycler for incubation, (1) provided with a heat block 1 for heating and maintaining the reaction vessel 4 for accommodating the reaction liquid 5, the heat block 1, lower layer heat block 3 and it is composed of two layers of an upper layer heat block 2, further comprising (2) by controlling the temperature of the lower heating temperature of the block 3 and the upper layer heat block 2 independently when incubating the reaction liquid 5, comprising a temperature control means for maintaining the temperature of the upper heating block 2 higher than the temperature of the lower layer heat block 3. Thus, does not occur coagulation of the reaction mixture the reaction vessel 4 in water and other ingredients in the 5, further since the temperature unevenness does not occur due to the installation position of the reaction vessel 4, reproducibility of PCR and other enzymatic reactions well it can be carried out.