First Draft Genome Assembly of Redlip Mullet (Liza haematocheila) From Family Mugilidae

Recent advances in next-generation sequencing (NGS) technologies have created opportunities to understand the genomic structure of higher-level organisms, and it has revolutionized the biological sciences and influenced aquaculture research. Discovery of marine organisms has progressed considerably due to NGS technologies, delivering a massive amount of genetic information quickly and affordably. Assembly of sequenced DNA reads to correct reference genomes is an essential task in genomic studies, for which diverse range of tools and algorithms have been developed. However, most of the genomes sequenced by NGS and other technologies are incomplete due to their large data size, time, and technical difficulties. High-throughput Illumina technology is exceptionally accurate, and thus, it is popular in the sequencing industry (Mavromatis et al., 2012). Also, single molecule real-time (SMRT) sequencing offered by Pacific Biosciences of California, Inc. (PacBio, USA) provides a longer average read length and high consensus accuracy (Yue et al., 2017); read length was nearly 27 kb under the PacBio Sequel System (Brown et al., 2014). Here, we present the draft genome of Liza haematocheila (L. haematocheila) (Figure 1A), which was sequenced using the current high-throughput sequencing platforms, PacBio and Illumina paired-end technologies, as a hybrid strategy. Open in a separate window Figure 1 (A) Photograph of redlip mullet, Liza haematocheila. (B) Estimation of L. haematocheila genome size based on 17-, 19-, and 21-mer count analysis. (C) Genome assembly quality assessment from Core Eukaryotic Genes Mapping Approach (CEGMA). (D) Repeat elements of the hybrid draft genome. (E) Benchmarks of the Benchmarking Universal Single Copy Orthologs (BUSCO). (F) Venn diagram of orthologous gene clusters between six fish lineages. (G) Phylogenetic tree developed by maximum likelihoood method. (H) Mapped proteins and mapping sites of annotated genes from Liza haematocheila to other species.