Multiplexed detection of biological agents using optical microchip sensors
2010
A multi-channel optical microchip sensor system suitable for
real-time, label-free detection of a wide range of biological
agents is presented. SpectroSensTM chips containing multiple high-precision planar Bragg gratings are exploited as lowcost,
robust refractive index sensors. Sensitivity to biological agents is conferred by functionalising individual sensing
regions with different antibodies selected against numerous targets of interest. Antigen binding to the surfaceimmobilised
antibodies results in localised changes in refractive index; upon laser-induced interrogation of the sensing
region via optical fibres, these antibody-antigen interactions manifest as increases in wavelength of light reflected from
the sensor chip. Real-time detection of multiple biological agents including bacterial cells/spores, viruses and toxins has
been demonstrated. Further improvements to sensor performance including physical and chemical methods are also
investigated. This multi-analyte capability highlights the potential use of this sensing technology in applications ranging
from bio-hazard detection for defence purposes to point-of-care clinical diagnostics.
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