Galactosylation of Cyclodextrins and Branched Cyclodextrins by α-Galactosidases
1994
Transgalactosylated derivatives of cyclodextrins (CDs) and glucosyl and maltosyl CDs (G1- and G2-CDs) were synthesized by α-galactosidases from coffee bean and Mortierella vinacea (M. vinacea). The structures of the transfer products were analyzed by FAB-mass, 13C-NMR and methylation.Coffee bean α-galactosidase transferred a galactosyl residue not only to side chains of G1-CDs and G2-CDs, but also directly to CD rings. M. vinacea α-galactosidase transferred a galactosyl residue only to side chains of G2-CDs.
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