Профили изменения экспрессии генов GPX1, RHOA и NKIRAS1 как потенциальные диагностические маркеры немелкоклеточного рака легкого

Lung cancer is distinguished by an extremely high prevalence and a high mortality rate due to its late detection and unavailability of methods for early diagnosis. Changes in gene expression were evaluated using quantitative RT-PCR. Expression of several chromosome 3 genes (specifically, RHOA, GPX1, and NKIRAS1) was shown to be increased 5 or more times in most samples of non-small cell lung cancer (NSCLC) and its histological subtypes, lung adenocarcinoma (AC) and squamous cell lung cancer (SLC). When a combination of three genes, RHOA, GPX1 and NKIRAS1, was used increased expression was detected in 85% (33/39) of NSCLC samples, including 78% (18/23) of SLC samples and 94% of AC (15/16) samples. Interestingly, when only samples from early-stage cancer (I/II) patients were analyzed, the sensitivity of the proposed set of genes was preserved and even somewhat increased.