Comparative Transcriptome Analysis of GR2 and GL05136 in Mature-stage of Sugarcane

Sugarcane 'GR2' and 'GL05136' belong to late and early maturing high sugar varieties. Transcriptome sequencing was performed on the mixed samples of the leaves and stems of the two varieties to compare the differences in gene expression and to screen and clone genes that may be involved in sugar synthesis. Two comparison methods of interspecific at the same period and intraspecific at different periods were adopted, and four comparison groups were set up in the two varieties. KEGG and GO databases were used to analyze the overall transcriptome and the gene differential expression in the related fields of sugar synthesis. The results showed that there were 97 gene sequences of 5 enzymes related to sugar synthesis and several high-expression genes whose functions were unknown. Subsequently, the function of the high-expression genes could be verified by building a transgenic sugarcane validation system, improving the molecular mechanism theory of sugar metabolism, developing high-sugar molecular markers and even building high-expression transgenic varieties.