Construction of a Genomic Library of Pythium guiyangense Su,A mosquito-killing Fungus

Objective:To construct a genomic library of Pythium guiyangense Su,strain 1938 GY.Methods:The digested genomic DNA with Sau3AI was inserted into pBluescript SK(-) plasmid which was digested with BamHI and treated with calf intestinal alkaline phosphatase.Next,the recombinant plasmids were transferred into E.coli DH5α.Results:A genomic DNA library was constructed which included 11520 clones with average insert sizes about 1.2 kb.Conlusions:The results of this experiment provide a source for screening genes of P.guiyangense,and lay basis for further work of genetic engineering of this fungus.