IN VITRO CYTOTOXICITY OF EMBLICA OFFICINALIS AGAINST DIFFERENT HUMAN CANCER CELL LINES

Cancer is a public health problem all over the world. Large number of plants and their isolated constituents have been shown to potential anticancer activity. Ethanolic whole plant extract of Emblica officinalis (syn. Phyllanthus emblica L.) showed in vitro cytotoxicity against different human cancer cell lines such as lung, neuroblastima, and colon. There was no growth of inhibition recorded against liver cancer cell line. Sulforhodamine B dye (SRB) assay was done for in vitro cytotoxicity test assay. The in vitro cytotoxicity was performed against five human cancer cell lines namely of lung (A-549), liver (Hep-2) colon (502713 HT-29) and neuroblastima (IMR-32). The activity was done using 100µg/ml of the extract. Against lung (A549) cell line plant extract showed 82% growth of inhibition. In case of liver (Hep-2) showed no activity reported, where as in case of colon 502713 cell line plant extract showed maximum activity. In case of HT-29 liver human cancer line and IMR-32 neuroblastima cell line plant extract showed 98% and 97% activity respectively.