Methods of induced pluripotent stem cells for clinicalapplication

Reprograming somatic cells using exogenetic geneexpression represents a groundbreaking step inregenerative medicine. Induced pluripotent stem cells（iPSCs） are expected to yield novel therapies withthe potential to solve many issues involving incurablediseases. In particular, applying iPSCs clinically holds thepromise of addressing the problems of immune rejectionand ethics that have hampered the clinical applicationsof embryonic stem cells. However, as iPSC research hasprogressed, new problems have emerged that need tobe solved before the routine clinical application of iPSCscan become established. In this review, we discuss thecurrent technologies and future problems of human iPSCgeneration methods for clinical use.