Multi-sensory (auditory and somatosensory) pre-pulse inhibition in mice.

Abstract We investigated the perception of two mechanoreceptive modalities alone and in combination: main effects and interaction between auditory and somatosensory stimulation in mice. Fifteen C57BL/6J mice between the ages of 1 and 6 months were tested three times each. Experimental design roughly followed published procedures using pre-pulse inhibition (PPI) of the acoustic startle response, except pre-pulses included vibration of the test chamber as well as soft sounds. Auditory pre-pulses were 80 dB broadband noises of 4, 9, 25, or 45 ms duration. Vibrations were of the same duration but of different frequencies (500, 460, 360, and 220 Hz). Pre-pulse inhibition increased with duration of the auditory pre-pulses, as expected. There was significant PPI to some but not all vibrotactile pre-pulses. Multimodal PPI was approximately additive (no significant auditory-by-somatosensory interaction). PPI increased more with age to somatosensory than to auditory pre-pulses. Future studies of multi-modal psychophysics in various mouse mutants could lend support to more mechanistic studies of neural specificity and possibly autism, tinnitus, and PTSD.