Hybridization properties of oligoarabinonucleotides

3′-Phosphoramidite derivatives of arabinocytidine, arabinoadenosine, and arabinouridine were prepared and used for the solid-phase synthesis of oligoarabinonucleotides (arabinonucleic acid, or ANA). Thermal denaturation analysis and gel mobility shift assays were used to investigate the interaction between ANA and complementary DNA and RNA. In general, the ANA/DNA and ANA/RNA complexes exhibited melting temperatures comparable to those of the corresponding DNA/DNA and DNA/RNA complexes. Thus the inversion of stereochemistry at the C2′ of ribonucleotides does not have a negative effect on interaction with natural sequences. In fact, in complexes with poly dT, oligoarabinoadenylates displayed greater hybridization affinity than oligoriboadenylates. In summary, we observed that (i) ara(Ap)7A interacted favourably with poly rU and poly dT, (ii) ara(Cp)7C formed a stable complex with poly rG; (iii) ara(Up)7U did not bind with complementary poly rA; and (iv) the mixed oligoarabinonucleotide ara(UCU UCC CUC UCC ...