Large serum protein of hepatitis B virus and its clinical application analysis.

OBJECTIVE To explore the value of large serum protein of hepatitis B virus (LHBs) in diagnosing type of hepatitis B and the pertinency with other markers of HBV.METHODS The enzyme linked immunosorbent assay (ELISA ) methods were used to examine LHBs,while Heat,Pres1Ag,and fluorescent quantitative PCR were used to detect the HBV DNA of 623 HBsAg positive serum samples.RESULTS The positive rates of LHBs and PreS1Ag were respectively 94.17% and 50.49%,the difference was statistically significant.Of the 270 HBeAg negative samples,the positive rates of HBV DNA and LHBs were respectively 49.26% and 44.07%,there was without statistical significance.LHBs optical density was positively correlated with HBV DNA copy level.CONCLUSION LHBs can be used as a new sensitivity indicator for reflecting the replication of HBV,its convenient operation is suitable for clinical application.