Antiplasmodial Activity of Methanol Extract and Pure Isolated Compound of Calophyllum bicolor P. F. Steven

Calophyllum bicolor (Clusiacea) is a big tree from Indonesian rain forest in Palangkaraya, Kalimantan . Calophyllum or bintagor is one of many sources of natural bioactive compounds that can be used in the fields of health and pharrmaceuticals. The aim of this research was to explore the ability of methanol extract of Calophyllum bicolor P.F. Steven to inhibit the growth of P lasmodium falciparum . The methanol extract was purified by colomn chromatographyc system, hexane – ethyl acetate was used as solvent with increasing polarity. One pure compound was obtained and was elucidated based on the 1D and 2D-NMR, COSY DEPT, HMBC and HMQC data and the isolated compound was identified as xanthone. Methanol extract showed growth inhibition against parasite P. falciparum with IC 50 5.2 ppm and pure compound have maximum inhibition at concentration 0.11 nMol respectively