Micro-dosage automatisé sur analyseur à transfert de l'azote α-aminé circulant

: An automated method for the determination of plasma free amino groups is described. The analysis is performed on a discrete computerized analyzer after plasma deproteinization using trichloracetic acid. The method is based on the formation of a complex between terminal NH2 radicals and 2,4,6-trinitrobenzene sulfonate (TNBS). The method is accurate (coefficient of variation = 2%), linear over a range of 0.2-7 mM/l and requires small analytic volumes; it also correlates well in the bovine with results from standard liquid chromatography (r = 0.953). With this method a single operator can determine free amino group contents of 50 samples per h.