Optimizing lipase production from isolated Burkholderia sp.

Abstract Lipase produced from microorganisms can be used in many industrial applications, such as detergent formulation, oil/fat degradation, pharmaceutical synthesis, biodiesel and cosmetics production. Due to the increase in demand for lipase, increasing attention has been paid to how to produce it efficiently and economically. In this work, lipase production from an isolated Burkholderia sp. C20 was enhanced by using the response surface methodology (RSM) to optimize the medium composition and the fermentation condition. The titer of lipase production was 16.7 U/ml under the optimal conditions with yeast extract of 2.175 g/l and olive oil of 5.54 ml/l. The lipase production was improved to 22.6 U/ml in a fermentor with an aeration rate of 1 vvm and a controlled pH at 6.0. The Burkholderia lipase exhibits good thermal and pH stabilities for 21 h incubation and the zymography analysis showed that the molecular weight of the lipase is about 23 kDa.