Spectrophotometric determination of lipid peroxidation end products

: Spectrophotometric assay of the final lipoperoxidation products based upon light absorption at 400 nm was developed. Relative content of these products in different phases of lipid extract was calculated as E400/E220 ratio. Data obtained by the technique developed were consistent with those of histochemical results. Appreciable correlation between the relative lipoperoxidation products content and age was demonstrated.