Isolation of Phosphate Solibulizing Actinomycetes from Forest Soils of Mahabubnagar District

The present study was carried out in order to establish a suitable protocol for the screening of phosphate solubilizing actinomycetes. 10 isolates with distinct morphology were isolated and purified on starch casein agar from forest soils of Mahabubnagar district, Andhra Pradesh. These isolates screened for phosphate solubilizing activity on modified starch casein agar which contains tricalcium phosphate as a sole „P‟ source. Out of 10 isolates, 4 isolates were showed the activity of phosphate solubilization. Among them two isolates were showed good activity. The solubilizing activity was maximum at pH 7.0 and with incubation period of 10 days. The isolate PSA-7, which is showing maximum activity of phosphate solubilization selected for 16S rRNA partial gene sequence and BLAST analysis revealed that the strain shown 98% similarity with streptomyces spp. The present study indicates that actinomycetes play an important role in the phosphate solubilization there by increases the soluble phosphate concentration, which is an essential for plant growth.