Techniques in Immunohistochemistry and Immunocytochemistry

Immunohistochemistry and immunocytochemistry are emerging as powerful tools to supplement the knowledge obtained by routine histological techniques. These have been significantly aided by technical advances in microscopy. The combination of immunolabeling techniques and microscopy now allows detailed insights into tissue and cellular architecture and is proving to be extremely valuable for understanding the micro architecture of the brain in health and disease. In this chapter, we discuss some of the considerations and techniques for immunohistochemistry of the brain and dissociated, cultured neurons from the hippocampus and cortex.