Compositional study of plasmalogens in clam (Corbicula fluminea) by TiO2/KCC-1 extraction, enzymatic purification, and lipidomics analysis

Abstract In this study, the plasmalogens in clam (Corbicula fluminea) were extracted by titanium dioxide (TiO2) fibrous silica nanosphere (KCC-1) extraction and phospholipase A1 hydrolysis. The molecular composition of plasmalogens was lipidomically analyzed using hydrophilic interaction chromatography mass spectrometry (HILIC-MS). The results showed that there was a total of 34 plasmalogen molecular species structurally elucidated. The quantitative results of linear regression models indicated that plasmenylcholine (14.24 μg mg–1) was the most abundant class, followed by plasmenylethanolamine (11.25 μg mg–1), and plasmenylserine (9.78 μg mg–1). Multivariate data analysis was applied to reduce data dimensions and circos map was used to provide an integrated and holistic view of correlation coefficients between the samples and plasmalogen molecular species. This method was validated to be accurate and sensitive in extraction and analysis of plasmalogens in clams.