Simultaneous determination of Salbutamol sulphate anddoxophylline in tablets by reverse phase liquid chromatography

A simple, isocratic reverse phase liquid chromatogr aphic method has been developed and validated for simultaneous determination of Salbuta mol Sulphate (SBS) and Doxophylline (DOX) present in tablet dosage forms. Chromatographic sep aration achieved isocratically on Luna C 18 column (5 µm, 150mm x 4.60mm) and Acetonitrile/KH 2 PO 4 buffer (40:60, v/v, pH 3.0 with OPA) as mobile phase, at a flow rate of 0.5 ml/min. Dete ction was carried out at 225 nm. Parameters such as linearity, precision, accuracy, recovery, sand ruggedness are studied as reported in the ICH guidelines. The retention time for SBS and DOX was found to be 3.14±0.015and 5.73±0.06 min respectively. Linearity for SBS and DOX was in the range of 4-20 μ g/ml and 400-2000 μ g/ml respectively. The mean recoveries obtained for SBS and DOX were 98.54and 98.79% respectively and RSD was less than 2. The correlation coefficients for all components are close to 1. The relative standard de viations for three replicate measurements in three concentrations of samples in tablets are alwa ys less than 2%. Developed method was found to be accurate, precise, selective and rapid for si multaneous estimation of SBS and DOX in tablets.