Detection of Prolactin Receptor in Skin of Inner Mongolia Alpas Cashmere Goat by Chemiluminscent Photography Western Blotting

Four skin samples were tacked from cashmere goats correspond-ing to four seasons. Total protein of the skin sample was extracted.Samplespotein was separated on SDS-PAGE.The gel was electrotransferred on to ni-trocellulose membrane, combined with prolactin receptor antibody and HRP-conjugated secondary antibody, acted on luminol chemiluminscent substrate,exposed against X--ray film, developed. Black spots were obtained. Accordingto the situation of the spot, the molecular weight of the prolactin receptor is 87kD. Expression quantity of the prolactin receptor is accordance with cashmeregrowth tendency.