PE-209 : Body Weight Gain Is Exaggerated by Diet Type on ad Libitum Feeding

Aims: Obesity raises the risk of morbidity from metabolic syndrome including dyslipidemia, diabetes mellitus and fatty liver disease. Weight loss is associated with lowering the risk of metabolic syndrome. To lose body weight, reduced-calorie diet and increased physical activity are required. We report foz/foz (11- bp truncating deletion in exon 8 of Alms1 gene) mice which has disordered appetite regulation fed with western diet, high fat diet and normal chow ad liblitum showed exaggerated body weight gain according to diet pattern. Methods: 4~5 weeks old foz/foz mice and wild type (WT) littermates were fed with western diet, choline-deficient high fat diet(CD-HFD) and normal chow for 12 weeks, ad liblitum. Every week, food intake and body weight were measured and after 12 weeks, metabolic indices were assessed by evaluating body weight, liver weight, the level of fasting glucose and aminotransferase. Results: Total amount of food intake showed no significant difference among western diet, choline-deficient high fat diet and normal chow in spite of different flavor and taste. Average calorie intake was 35% higher in WD and CD-HFD compared with normal chow. However, body weight gain was 75% higher in WD and 49% higher in CD-HFD. AST was 10 times higher in WD and CD-HFD compared with normal chow. All three groups showed glucose intolerance. Conclusions: To reduce the risk of metabolic syndrome associated with obesity, reduced calorie intake and increased physical activity is mandatory. However, to keep reduced calorie intake is challeged while restriction of food intake only. This finding showed that western diet induced most exaggerated body weight gain and body weight gain is influenced more by diet pattern rather than calori itself. Finally, changing behavioral eating pattern is recommended to keep the reduced body weight and improve NASH.