Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intracellular 1-beta-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular lymphoma cell line.
2009
A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular lymphoma cell line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 gm L −1 for araCTP and of 0.01 gm L −1
Keywords:
- Triple quadrupole mass spectrometer
- High-performance liquid chromatography
- Deoxycytidine triphosphate
- Liquid chromatography–mass spectrometry
- Chromatography
- Selected reaction monitoring
- Chemistry
- Cytidine triphosphate
- Arabinofuranosylcytosine triphosphate
- Tandem mass spectrometry
- Follicular lymphoma
- Molecular biology
- Cell culture
- Intracellular
- Beta (finance)
- lc ms ms
- Correction
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