Identification of three common sandfies in southern Xinjiang with multiplex PCR

Based on the variable part of mtDNA COⅠ gene sequence, a multiplex PCR method was developed for the identification of the three common sandflies(Phlebotomus longiductus, Ph. wui, and Ph. alexandri) in southern Xinjiang. The results demonstrated that this multiplex PCR method was reliable, and could be used to identify the three Phlebotomus species. The PCR product of CO Ⅰ gene from Ph. longiductus, Ph. wui and Ph. alexandri was 248, 632,and 395 bp, respectively.