Retrospective molecular study on canine hepatozoonosis in Slovakia – Does infection risk for dogs really exist?

Abstract This study provides the very first evidence of Hepatozoon canis infection in dogs from Slovakia, a Rhipicephalus sanguineus sensu lato free area. In total, 297 red foxes ( Vulpes vulpes ) and 293 dogs, from three regions of Slovakia, were screened for the presence of H. canis using a conventional 18S rRNA polymerase chain reaction (PCR). Genomic DNA of this blood parasite was detected in 51 (17.1%) foxes from all sampling regions in Slovakia, whereas the overall prevalence in examined dogs was significantly lower, only 1.0%. Identity between nucleotide sequences from Slovak foxes and dogs ranged from 97.3 to 100%. Phylogenetic analysis showed that H. canis isolates from Slovakia are divided into two clusters, suggesting the coexistence of strain variants with unknown pathogenicity which needs to be further explored.