Peptide Synthesis in Organic-Aqueous Media Catalysed by α-Chymotrypsin Immobilised Over Different Supports

Abstract Peptide synthesis has been carried out using α-chymotrypsin (α-CT) immobilised on different supports (agarose, two polyethylene-g-co-2-hydroxyethyl methacrilate (PE/HEMA) copolymers, and two soluble acrilic microgels), using different organic-aqueous media. Fairly good yields were obtained with all of them, and their reutilisation at least two times was excellent. The influence of the physicochemical properties of supports and solvents in the yield in peptide synthesis is discussed.