Succession of microbial community in physical simulation of indigenous microorganism flooding

Activating nutrients was designed for Zhongyi block in Shengli Oil Field according to the character of stratal water, which could stimulate indigenous microorganisms effectively,and keep Sulfate Reducing Bacteria under 50 cells·mL-1,where acetate and methane produced by microbe were 346 mg·L-1 and 5%,respectively.15 different genuses of indigenous microorganisms were detected by Denaturing Gradient Gel Electrophoresis and sequencing,most of which were thermophilic anaerobes.Some bacteria could produce low molecular weight organic acids,CO2 and H2,which could be used as substrates by other bacteria and archaea,all of these microorganisms form an entire ecology system under physical simulation conditions.