Alterations of MCF-7 human breast cancer cell after prostaglandins PGA1 and PGF2α treatment

Treatment of monolayer cultures of MCF-7 cells with prostaglandins PGA1 and PGF2α inhibited cell proliferation, reduced the rate of labeled precursor incorporation into DNA, RNA, and protein, and induced morphological changes in a dose-dependent manner. The rate of [3H]thymidine incorporation was increased by PGA1 at l0–10–l0–8M, while a sharp decrease was observed at 10–6–10–4 M(p 2α inhibited [3H]thymidine incorporation at all concentrations tested. Similar results were obtained for [3H]uridine incorporation with both PGs. PGA1 inhibited [3H]leucine incorporation at 10–4M, but increased incorportion at 10–10–10–6M. At the ultrastructural level, neither PG induced morphological alterations at 10–12–10–8M. However, at 10–6–10–4 Mboth PGA1 and PGF2α diminished the number and size of cell surface projections; some cells appeared to completely lack microvilli. Disorganization of mitochondrial cristae and increased electron density of the matrix were also evident