[Activation of ERK1/2 and Elk1 in A549 cells induced by crocidolite].

Objective To investigate the role of extracelluar signal regulated kinase (ERK) 1/2 and Elk1 in pulmonary disease induced by crocidolite asbestos fiber. Method Western blotting and Immunoprecipitation were used to detect the expression of phosphorylated ERK1/2 and Elk1 in human bronchial airway A549 cell line stimulated by crocidolite. Results The expression of phosphorylated ERK1/2 and Elk1 were striking higher than those of the control, the differences were significant (P0.05). Conclusion Phosphorylated ERK1/2 and Elk1 probably involved in the process of the diseases induced by crocidolite.