Study of genetic diversity of Iranian indigenous buffalo populations using microsatellite markers
2019
Indigenous buffalo breeds represent a unique genetic resource, and
understanding their variability, population structure and breeding units is
important for their sustainable conservation. In the present study the
genetic structure of Iranian buffalo populations was analyzed using ten
microsatellite markers. Two hundred hair samples were collected and DNA was
extracted using modified salting out method. After Polymerase Chain Reaction
(PCR), the PCR products were electrophoresed using 9% polyacrylamide gel.
Fifty- nine alleles were observed for all the loci. The average number of
alleles was 5.90 and the effective average number of alleles was 4.86. The
high level of mean heterozygosity index between three populations indicate
that the genetic diversity is high in within and between populations. The
mean of polymorphism information content (PIC) value for all loci was 0.70.
The FST value for the total loci was 0.01, indicating a very low level of
genetic structure among populations. The genetic structure AMOVA analysis
showed that about 3% of the total genetic variation was explained by
population differences and 97 percent was corresponded to differences among
individuals. The obtained results at the present study indicated that
characterization of genetic diversity by employing molecular tools is a
prerequisite in developing strategies for conservation and utilization of
buffalo genetic resources.
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