Study of genetic variability of chicken growth hormone gene in broiler strain (vencobb-400) by PCR-RFLP

Present study to estimate the genetic variability of Chicken growth hormone gene in broiler strain (Vencobb-400). In order to study and investigate chicken growth hormone gene polymorphism, blood samples were collected from 150 individuals of broiler and Genomic DNA was extracted from whole blood using spin column method as per manufacture protocol with slight modification. The purity of extracted genomic DNA was determined by 0.8% agarose gel electrophoresis. DNA fragment with 776 bp at Chicken growth hormone gene was amplified using Polymerase Chain Reaction (PCR). The PCR product was digested with restriction enzyme MspI and subsequently electroforesed on 8% polyacrylamide gel with ethidium bromide. In this population, heterozygosis was estimated 0.28 and obtained genotypes frequency of MspI enzyme on 776 bp fragment for AA, AB and BB genotype were .22, .28, and .50, respectively. The gene frequency of A and B alleles were .39 and .61, respectively.