Genetic Dissimilarity in Conyza sumatrensis Revealed by Simple Sequence Repeat (SSR) Markers

2020 
In view of the rapid evolution of Conyza sumatrensis populations resistant to glyphosate, it is necessary to understand the genetic diversity aimed to improve strategies for managing this weed. We investigated the genetic dissimilarity among 15 biotypes of C. sumatrensis from different geographic regions using microsatellite loci. The biotypes, were cultivated in a greenhouse to obtain vegetal material for DNA extraction. Nineteen microsatellite markers (SSR), were developed for C. sumatrensis biotypes. The genetic dissimilarity was estimated by the Jaccard coefficient (JC) and the biotypes grouped by the UPGMA method. The results demonstrated a high dissimilarity (JC = 7.14 to 82.62) of the analyzed material, with the biotypes forming five groups, being one group formed just by the susceptible biotype and in the others grouped by biotypes from distinct locations in the same group The high genetic diversity of C. sumatrensis indicates that the biotypes may show different responses to different management strategies, and that the mechanisms of resistance to herbicides and characteristics of evolution of populations due to adaptability may be some of the factors involved in the genetic variability of the species. Keywords: polymorphism; SSR; tall fleabane; genetic variability RESUMO: Tendo em vista a rapida evolucao das populacoes de Conyza sumatrensis resistentes ao glifosato, e necessario entender a diversidade genetica com vistas a melhorar as estrategias de manejo dessa planta daninha. Diante do exposto, objetivou-se com este trabalho investigar a dissimilaridade genetica entre 15 biotipos de C. sumatrensis de diferentes regioes geograficas usando marcadores moleculares microssatelites. Os biotipos foram cultivados em casa de vegetacao, para obtencao de material vegetal para extracao de DNA. Dezenove marcadores microssatelites (SSR) foram desenvolvidos para os biotipos de C. sumatrensis. A dissimilaridade genetica foi estimada pelo coeficiente de Jaccard (JC), e os biotipos, agrupados pelo metodo UPGMA. Os resultados demonstraram alta dissimilaridade (JC = 7,14 a 82,62) do material analisado, com os biotipos formando cinco grupos. A alta diversidade genetica de C. sumatrensis indica que os biotipos podem apresentar distintas respostas a diferentes estrategias de manejo e que os mecanismos de resistencia a herbicidas podem ser um dos fatores envolvidos na variabilidade genetica das especies. Palavras-chave: polimorfismo; SSR; buva; variabilidade genetica
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