Partial correlation of the genetic and physical maps of bacteriophage Mu

Abstract λpMu phage particles that carry varying amounts of the Mu genome have been used to correlate the genetic and physical maps of phage Mu DNA. Two complementary techniques, restriction enzyme fragment analysis and electron microscope heteroduplex mapping, have been employed to identify the segments of Mu DNA present in the λpMu particles whose genetic complement had been determined by Mu amber mutant marker rescue. These analyses permitted us to assign a physical location of genes A to lys on one end (immunity end) of the Mu genome, and genes L to S on the other end (variable end). None of the hybrids described here included genes E to K which lie in the middle part of Mu DNA.