Principle of Surface Plasmon Resonance (OneStep)

This chapter details the basic principle of SPR with appropriate components needed for the functioning of the system PioneerFE from Sartorius (previously Fortebio). SPR technology can be used to probe for affinity and kinetics between a range of molecules from small-molecule fragments to virus particles without the need for labels for detection. Binding interactions are monitored in a biosensor that is comprised of a thin gold-coated glass slide.