Analysis performance of biological toxicity testing MTOXPlate using microplate

Biological toxicities of heavy metals(Hg2+,Cd2+,Cr6+ and Pb2+),phenolic compounds(2,4-DCP,2-CP,4-CP,2-NP and 4-NP),surface water and wastewater were determined with the MTOXPlate based on 11 genetically diverse microorganisms and the analysis endpoint of dehydrogenase activity inhibition.The results showed that the MTOXPlate had good performance for toxicity analysis of heavy metals and phenolic compounds.The EC50 values of Hg2+,Cd2+,Cr6+,Pb2+,2,4-DCP,2-CP,4-CP,2-NP and 4-NP detected by the MTOXPlate were 0.617,21.05,35.21,11.22,27.26,64.29,44.19,85.89 and 33.84 mg·L-1,respectively.The dehydrogenase activity inhibition of surface water was less than 20%.Moreover,the EC50 values of sewage and dyeing wastewater were 62.19% and 16.42%,respectively.Thus,the biological toxicity testing technology MTOXPlate could reflect the actual toxicity of surface water,sewage and dyeing wastewater.