Chromosome instability and expression of BRAF, TERT and P53 in macrophage murine cell line (J774-1)

Background Cancer origin is closely linked with mutations and changes in the chromosome structure, making important such cytogenetics studies [1]. Macrophage murine cell line J774-1 is derived from a reticulum cell sarcoma and presents various characteristics of primary macrophages as synthesis and lysozyme secretion, phagocytosis, Fc receptor expression [2,3]. This work aimed to analyze the fundamental number and the constitutive heterochromatin using the staining with the flurochrome Hoechst 33258. In order to undertand possible changes in gene expression pattern, it was also quantified the levels of expression of the following important classes of genes: (A)-Genes responsible to promoting cell proliferation as BRAF and Telomerase (TERT); (B)-The P53 gene responsible by tumor suppressing.