Determination of linezolid in human plasma by HPLC

AIM To establish a HPLC method for the determination of linezolid in human plasma.METHODS The analytical column was packed with Eclipse XDB-C_(18),the mobile phase was methanol-water(45:55,V/V),the flow rate was 1.0 mL·min~(-1),and the detection wavelength was 254 nm.Proteins in plasma samples were precipitated by 70%perchloric acid,and detected at 254 nm.RESULTS The liner range of linezolid in human plasma was 0.25-50 mg·L~(-1)(r =0.999 8).The limit of detection was 0.25 mg·L~(-1).The intra-day and inter-day RSD for assaying the plasma sample containing three concentration of linezolid were both lower than 10%.The relative recovery were 96.40%, 103.24%and 100.33%,respectively.The absolute recovery were more than 75%.CONCLUSION The HPLC method to determine linezolid in plasma is simple,high selectivity and sensitivity.