Expression, purification and biophysical characterization of recombinant Streptomyces violaceoruber phospholipase PLA2 overproduced in Pichia pastoris

S. Y. Filkin,N. V. Chertova,A.A. Zenin,A. V. Lipkin,A. A. Sichev,D. S. Bityak,E. G. Sadykhov,Vladimir Popov,A. N. Fedorov

Expression, purification and biophysical characterization of recombinant Streptomyces violaceoruber phospholipase PLA2 overproduced in Pichia pastoris

2020

S. Y. Filkin
N. V. Chertova
A.A. Zenin
A. V. Lipkin
A. A. Sichev
D. S. Bityak
E. G. Sadykhov
Vladimir Popov
A. N. Fedorov

AbstractAim: The main purpose of this work was to develop new protocols for high yield purification of secretory phospholipase A2 (PLA2) and to investigate its biophysical properties.Materials and ...

Keywords:

Chemistry
Biochemistry
Pichia pastoris
Streptomyces violaceoruber
Recombinant DNA
Phospholipase
Phospholipase A2
Secretory Phospholipase A2

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