Differentiation of Hippelates Pusio, H. Bishoppi and H. Pallipes (Diptera: Chloropidae) by Electrophoresis

Hippelates pusio Loew, H. bishoppi Sabrosky, and H. PALLIPES (Loew) (Diptera: Chloropidae) from North Carolina were examined by starch gel electrophoresis for 5 enzymes: esterase, α-glycerophosphate dehydrogenase, “leucine” aminopeptidase, NAD dependent malic dehydrogenase, and aldehyde oxidase. Both similarities and differences in electrophoretic patterns among the species were noted. The esterase and aminopeptidase patterns from larval extracts apparently were distinguishing characteristics of all 3 species. The other enzymes were less species-specific.