Effects of glycolytic pathway on generation of regulatory T cells during Schistosoma japonicum infection

: [摘要] 目的 探索日本血吸虫感染过程中糖酵解途径对小鼠调节性T (Treg) 细胞数量和功能的影响。方法 建立日 本血吸虫感染小鼠模型, 用糖酵解抑制剂2-Deoxy-D-glucose (2DG) 或PBS对日本血吸虫感染小鼠进行6次腹腔注射后, 分离脾脏细胞和肠系膜淋巴结, 采用流式细胞术 (FCM) 检测分离得到的细胞中Glut1+CD4+ T细胞以及Treg细胞比例。结 果 未感染组小鼠脾脏 (43.58%±2.50% vs. 21.15%±0.96%; t = 8.834, P < 0.01) 和肠系膜淋巴结中Glut1+CD4+ T细胞比例 (38.97%±1.97% vs. 28.40%±2.11%; t = 3.662, P < 0.05) 均显著高于感染日本血吸虫8 周小鼠, 但未感染组小鼠脾脏 (6.83%±0.21% vs. 13.30%±0.35%; t = 15.65, P < 0.01) 和肠系膜淋巴结中Treg细胞比例 (8.26%±0.15% vs. 14.37%±0.44%; t = 13.14, P < 0.01) 均显著低于感染组小鼠。感染小鼠给与2DG腹腔注射后, 脾脏 (15.50%±0.76% vs. 13.07%±0.15%; t = 3.130, P < 0.05) 和肠系膜淋巴结中Treg细胞比例 (17.00%±0.41% vs. 13.83%±0.18%; t = 6.947, P < 0.01) 显著高于给与 PBS注射小鼠。结论 糖酵解途径抑制了日本血吸虫感染小鼠Treg细胞分化。. METHODS: A S. japonicum -infected mouse model was established, and C57/BL6 male mice infected with S. japonicum were subjected to intraperitoneal injections of with the glycolytic inhibitor 2-Deoxy-D-glucose (2DG) or PBS for 6 times, and then the cells from spleen or mesenteric lymph nodes (LNs) were isolated and analyzed by flow cytometry (FCM) to detect the percentage of Glut1+CD4+ T cells and Treg cells. RESULTS: The proportions of Glut1+CD4+ T cells in the spleen (43.58%±2.50% vs. 21.15%±0.96%; t = 8.834, P < 0.01) and mesenteric LNs (38.97%±1.97% vs. 28.40%±2.11%; t = 3.662, P < 0.05) were higher in the normal mice than those in the infected mice, and the percentages of Treg cells in the spleen (6.83%±0.21% vs. 13.30%±0.35%; t = 15.65, P < 0.01) and LNs (8.26%±0.15% vs. 14.37%±0.44%; t = 13.14, P < 0.01) were lower in the normal mice than those in the infected mice. In addition, the proportions of Treg cells in the spleen (15.50%±0.76% vs. 13.07%±0.15%; t = 3.130, P < 0.05) and LNs (17.00% ±0.41% vs. 13.83%±0.18%; t = 6.947, P < 0.01) were higher in the infected mice injected intraperitoneally with 2DG than those in the infected mice injected intraperitoneally with PBS. CONCLUSIONS: Glycolytic pathway inhibits Treg differentiation in the spleen and mesenteric LNs of S. japonicum-infected mice.