Inhibition of 22 kinds of Chinese herbs on α-glucosidase in vitro.

Objective: To explore the inhibition of 22 kinds of Chinese herbs on α-glucosidase and screen Chinese herbs which have greater inhibition.Methods: The experiment was carried out with maltose as substrate,and then established optimal reaction conditions.The inhibition of 22 kinds of Chinese herbs and acarbose on α-glucosidase were tested by the enzyme reaction system.Here,acarbose was used as a positive control group.Results: All of the 22 kinds of Chinese herbs could inhibit the activity of α-glucosidase,and Sangye,Sangbaipi,Diyu,Qingguo and Wubeizi were the fi rst fi ve,the inhibition rates were 63.19%,76.55%,70.03%,78.18% and 92.83% respectively at the concentration of 500mg/ml.The inhibition of Wubeizi was the strongest,and when its concentration reached 1000mg/ml,the inhibition rate increased to 95.44%,which was similar to the effect of 5mg/ ml acarbose.Conclusion: Compared with the other Chinese herbs,Sangye,Sangbaipi,Diyu,Qingguo and Wubeizi had stronger inhibition on α-glucosidase in vitro.