In vivo Pharmacokinetics of Notoginsenoside R1 in Ischemia Rats After Acute Myocardial Infarction

Objective: To establish an HPLC-UV method for determining pharmacokinetic difference of notoginsenoside R1 between normal rats and ischemic rats. Methods: 48 male SD rats were randomly divided into normal group and acute myocardial ischemia( AMI)model group induced by pituitrin and each group was classified into high,middle and low-dose of groups with notoginsenoside R1( 200,100 and 50 mg/kg) respectively. Blood samples were collected at different points in time after they were administered once by gavage and separated by Waters symmetry C18column( 250 mm × 4. 6 mm,5 μm) under the detective wavelength 203 nm,the mobile phase was acetonitrile-water with icariin as the internal standard and the pharmacokinetic parameters were calculated by DAS 2. 0. Results: Notoginsenoside R1 had good linearity in the ranges of 0. 2 ~ 125 μg/m L( R2= 0. 9997) with SNR 1 ∶ 3 and the lowest detection limit was0. 053 μg/m L,the extraction rate,RSDs of within-day and between-day,specificity,accuracy and precision accorded with the requirement of bio-sample pretreatment. Compared to the normal group,AUC0-tand AUC0-∞was significantly increased( P 0. 01) and the terminal half-life was prolonged markedly( P 0. 01) in AMI group. Conclusions: The method is simple,accurate and had high specificity and sensitivity,that could be applied in quantitative determination of notoginsenoside R1 and research of pharmacokinetics; the relative bioavailability of notoginsenoside R1 is increased significantly in AMI group,which indicates that notoginsenoside R1 has better effect in model rat.